TABLE 2.
Primers used in this study
| Description of target | Product length (bp) | Product Tm (°C)a | Primer names | Primer sequences |
|---|---|---|---|---|
| RD9 present | 51 | 76.3 ± 0.1 | RD9 Present Forward | TTTCGAGCCGTAAATTACTGTG |
| RD9 Present Reverse | GAGCATTCTCGCTCCGAAT | |||
| RD1 deleted | 226 | 86.2 ± 0.6 | RD1 Deleted Forward | GGATTTGACGTCGTGCTTCT |
| RD1 Deleted Reverse | TTCAACGGGTTACTGCGAAT | |||
| RD4 present | 55 | 77.8 ± 0.2 | RD4 Common Forward | AGAAGCGCAACACTCTTGGA |
| RD4 Present Reverse | CATGCGCCCTATTTGATCTC | |||
| RD4 deleted | 94 | 83.0 ± 0.2 | RD4 Common Forward | AGAAGCGCAACACTCTTGGA |
| RD4 Deleted Reverse | TTGCTGAAAAATGGCTATTGA | |||
| Mycobacterial 16S rRNA | 78 | 79.3 ± 0.1 | Genus Control Forward | CAACGCGAAGAACCTTACCT |
| Genus Control Reverse | TGCACACAGGCCACAAGGGA |
a
The product melting temperatures (Tm) show the mean ± the 95% confidence interval.