FIG. 1.
Example of a sensitivity assay performed on the SmartCycler thermocycler with 10-fold serial dilution of the genomic DNA purified from strain J99. (A) The fluorescence signal was from FAM dye of the 23SScWT-specific Scorpion primer. (B) Second derivative curves of fluorescence for FAM dye. (C) Standard curve plotting log concentrations versus CTs, calculated linear fit equation, and correlation coefficient. (D) Fluorescence curves for Texas red. (E) Fluorescence curves for Cy3 dye. (F) Fluorescence curves for Cy5 dye.