TABLE 2.
Outcome and no. of specimens (total) | Result bya:
|
||
---|---|---|---|
Culture and Etest | PCR-RFLP | Scorpion PCR | |
Concordance of all 3 methods (238 [92%]) | |||
184 | Negative | Negative | Negative |
34 | S | S | S |
15 | R | A2143G | A2143G |
5 | R | A2142G | A2142G |
Mixtures not detected by culture and by PCR-RFLP (3) | |||
2 | R | A2142Cb | A2142C + S |
1 | R | A2143G | A2143G + S |
Default of PCR-RFLP (2) | |||
1 | S | Negative | S |
1 | R + S | S | A2142G + S |
Default of PCR methods (1) | |||
1 | S | Negativec | Negativec |
Default of culture (12) | |||
7 | Negative | S | S |
4 | Negative | A2143G | A2143G |
1 | Negative | A2142G + S | A2142G + S |
Detection only by Scorpion PCR (3) | |||
3 | Negative | Negative | S |
S, susceptible; R, resistant.
Detected by sequencing.
Negative because of the presence of PCR inhibitors.