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. 2008 May 7;46(7):2320–2326. doi: 10.1128/JCM.02352-07

TABLE 2.

Results obtained by culture, PCR-RFLP, and Scorpion PCR from 259 biopsy specimens

Outcome and no. of specimens (total) Result bya:
Culture and Etest PCR-RFLP Scorpion PCR
Concordance of all 3 methods (238 [92%])
    184 Negative Negative Negative
    34 S S S
    15 R A2143G A2143G
    5 R A2142G A2142G
Mixtures not detected by culture and by PCR-RFLP (3)
    2 R A2142Cb A2142C + S
    1 R A2143G A2143G + S
Default of PCR-RFLP (2)
    1 S Negative S
    1 R + S S A2142G + S
Default of PCR methods (1)
    1 S Negativec Negativec
Default of culture (12)
    7 Negative S S
    4 Negative A2143G A2143G
    1 Negative A2142G + S A2142G + S
Detection only by Scorpion PCR (3)
    3 Negative Negative S
a

S, susceptible; R, resistant.

b

Detected by sequencing.

c

Negative because of the presence of PCR inhibitors.