FIG. 6.

Detection of gB mRNA in BCBL-1 cells. Total RNA was extracted from transiently transfected BCBL-1 cells and first-strand cDNA was used for PCR using primers. Shown are wild-type gB (gBwt) (A), universal gB primers amplifying both wild-type and codon-optimized gB (gBUNI) (B), and gB carboxy primers amplifying the 3′ end of gB (gBCarb) (C) and GAPDH primers producing an 80-bp amplicon. The levels of gB transcripts obtained after densitometric analysis and normalization with GAPDH are shown aligned below for each sample.