FIG. 5.
γH2AX levels are reduced in Tax-expressing cells following DNA damage. (A) Immunofluorescent staining of γH2AX (red) and DAPI staining (blue) in CREF-Neo (Neo) and CREF-Tax (Tax) cells at 30 min after exposure to IR (10 Gy). Images were taken at a magnification of ×63. (B and C) Western blot analysis of γH2AX, total H2AX, actin, H4, and Tax in extracts prepared from CREF-Neo and CREF-Tax (B) or CEM and HuT102 (C) cells at the indicated times following exposure to IR. (D) Densitometric analysis of the data shown in panel B was used to calculate the percentage of γH2AX at each time point using the formula (γH2AXA/total H2AXA)/(γH2AXB/total H2AXB) × 100, where A represents densitometric volumes for each individual time point and B represents densitometric volumes of CREF-Neo cells 30 min after gamma irradiation.