TABLE 1.
Effects of PZF and different nucleosides on the replication of SVpzfa
| Addition | Virus yield (PFU/ml) |
|---|---|
| None | 4.0 × 106 |
| PZF | 1.8 × 108 |
| PZF + adenosine | 1.3 × 108 |
| PZF + guanosine | 1.7 × 108 |
| PZF + uridine | 3.2 × 106 |
| PZF + cytidine | 3.0 × 106 |
a
Mosquito cells were infected with SVpzf at an MOI of approximately 3 PFU/ml. After adsorption, cultures were refed with medium; PZF, adenosine, guanosine, uridine, and cytidine were then added to the cultures as indicated. PZF was added to a final concentration of 1 μM, and the nucleosides were added to a final concentration of 150 μM. After 24 h, media were harvested and assayed for infectious virus on CEFs.