TABLE 3.
Effects of the different nucleosides on the yields of SVstd and SVcpca
Addition | Yield of virus (PFU/ml)
|
|
---|---|---|
SVstd | SVcpc | |
None | 4.0 × 108 | 3.5 × 108 |
Adenosine | 3.6 × 108 | 5.0 × 108 |
Guanosine | 1.3 × 108 | 3.0 × 108 |
Uridine | 6.1 × 108 | 2.7 × 106 |
Cytidine | 6.4 × 108 | 3.9 × 106 |
Mosquito cells were infected with either SVstd or SVcpc at an MOI of approximately 2 PFU/cell. After adsorption of virus for 1 h, cultures were fed with 2 ml of medium; adenosine, guanosine, uridine, and cytidine were then added to a final concentration of 200 μM as indicated. Media were harvested 24 h after infection and assayed for infectious virus on CEFs.