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Neutralization of the HSV-1 WT and gC/gE mutant viruses by antibody. The WT virus or the gC/gE mutant virus was incubated for 1 h at 37°C with 1% serum treated with EDTA to inactivate complement. Comparing antibody-mediated neutralization of the gC/gE mutant with that of the WT virus: HIV negative, P = 0.18; CD4 <200/μl, P < 0.01; CD4 200 to 500/μl, P < 0.06; and CD4 >500/μl, P < 0.04; all four groups combined, P < 0.0001.
