FIG. 3.

The gC/gE mutant and WT viruses express comparable concentrations of HSV-1 glycoproteins on the virion. Purified gC/gE mutant virus and WT virus were evaluated for VP5, gB, gC, gD, gE, gH, gL, and gI expression by Western blotting and densitometry analysis to compare relative glycoprotein concentrations. Each lane contains 1 × 10⁶ PFU of the WT or gC/gE mutant virus. The number associated with each gel represents the ratio of the density of the glycoprotein band above the number to the density of the VP5 band below the number. The results are representative of two separate experiments that yielded similar findings.