TABLE 3.

Vaccinia virus ORFs recognized by CD4 T cells from 82 to 100% subjects (9 to 11 of 11)

ORFa	Lengthb	Virionc	Promoterd	Human CD8e	Variola identityf	IgG prevalenceg	Comment(s)h
A3L	644	Yes	L	Yes	99	0	Capsid core
A10L	891	Yes	L	Yes	97	10	Capsid core
D13L	551	Yes	L	No	99	8	Membrane scaffold
H3L	324	Yes	E/L	Yes	97	9	IMV envelope, heparin binding
L4R	251	Yes	L	No	100	4	ssDNA/ssRNA binding; microtubule associated
WR148	725	Yes	L	No	92	10	Fragment of homolog of cowpoxvirus A type inclusion

^aNomenclature from strain Copenhagen, except for WR148, which is from strain Western Reserve; actual PCR amplicons for protein expression were from strain WR (see text). WR148 is related to vaccinia virus Copenhagen gene A26R (see reference 50 for details).

^bPredicted length in amino acids from genomic information (50).

^cYes indicates detection by mass spectroscopy in one or more studies of purified virions (14, 62, 80).

^dBased on personal communication with E. Lefkowitz, C. Hendrickson, and C. Wang, University of Alabama at Birmingham.

^eBased on a recent review (43).

^fAnalysis for vaccinia virus Copenhagen (or WR for WR148) and variola Bangladesh 1975 was performed using the methods described in reference 50.

^gPercentage of the 10 subjects in this report with IgG reactivity to the listed ORF. Subject 11 had no serum samples available for analysis.

^hFrom reference 50 and a survey of the literature. IMV, intracellular mature virion; ssDNA, single-stranded DNA; ssRNA, single-stranded RNA.