FIG. 2.

Vaccine protection against i.p. vaccinia virus challenge. BALB/c mice (n = 8/group) were immunized once i.m. with 10¹⁰ vp of rAd35-Empty (sham vaccine) (A), the mixture of all four rAd35 vaccine vectors (each at 2.5 × 10⁹ vp) (B), rAd35-A27L (C), rAd35-A33R (D), rAd35-B5R (E), or rAd35-L1R (F). At week 4 following immunization, mice were challenged systemically by the i.p. route with a lethal dose (2 × 10⁸ PFU) of vaccinia virus. Body weights were determined daily, and mice were euthanized if they lost >20% of their body weight based on institutional animal guidelines. Asterisks indicate mortality.