FIG. 3.
The cslASc gene affected aerial-hyphal development. (A) Multiple closely spaced sporulating hyphae of the mutant often emerged from one supporting hypha. Usually, the sporulating hyphae of wild-type M145 were well separated. (B) Spores of the mutant and wild-type M145 stained with PI. Spores of sporulating hyphae of the mutant contained DNA with the same appearance as that of sporulating wild-type M145. DNA-free spores of the mutant were rare (R2YE solid medium; analysis of the mutant was delayed compared to the wild type until spore chains could be detected).