TABLE 1.

Bacterial strains and oligonucleotides

Strain or oligonucleotide	Characteristics	Source
Strains
E. coli
DH5α	supE44 lacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1	31
ET12567/pUZ8002	dam dcm hsdS cat tet tra neo RP4	21
S. coelicolor
M145	SCP1− SCP2−	1
XE	M145ΔcslASc::Hygr cassette	This work
Oligonucleotide primers for PCR amplification
pcslAsc5	5′ AATGGATCCTCACACTCCCGGTCGGCAGG3′
pcslAsc6	5′ TCTAGATTACATATGTCATTCCCCCCACACGCGGG3′
pcslAsc7	5′ ATTAGATCTGGTTCTGGTGGTTCTGGTATGACGTCGACGCCGACGGG3′
pcslAsc8	5′ TTATGCGGCCGCTTATCATTCCTTACGTCCCCCAAG3′
pramS1	5′ AGTAGATCTGCCGGTCGTGTCGTAGGTG3′
pramS2	5′ TCTAGATTACATATGTCCGAGGTGCGGAACGCAC3′
pdivIVA1	5′ AGTGGATCCGAGGACGTGCGGAACAAGCAG3′
pdivIVA2	5′ AGTGAATTCCTCGTCGATCAGGAACCCGCG3′
pcslAsc3	5′ AGTGAATTCTTCCTCACCTCCTTCGTGCCC3′
pcslAsc4	5′ AGTAGATCTCGAGCCGCCGATCTGCTTGAG3′