FIG. 5.

Kinetics of colony formation from sleB spoVF spores and ger3 sleB spoVF spores. Spores of strains FB122 (sleB spoVF) (A) and PS4138 (ger3 sleB spoVF) (B) were prepared as described in the legends to Fig. 3 and 4, purified, and applied to LB medium plates; the plates were incubated at 37°C, and colonies that appeared over a 7-day period were counted every 24 h. Since colony formation from spores requires spore germination, the colony formation was expressed as percent spore germination, with the total colonies formed in 7 days defined as 100%. The DPA concentrations in the medium in which the spores were prepared were as follows: ○, no DPA; ▵, 10 μg/ml DPA; and •, 300 μg/ml DPA.