FIG. 8.

NMR spectra of small molecules extracted from wild-type and spoVF spores. Spores of strains PS533 (wild-type) (A), FB108 (ger3 spoVF) (B and C), FB122 (sleB spoVF) (D), and PS4138 (ger3 sleB spoVF) (E and F) were prepared with 300 μg/ml DPA (B and E) or without DPA (A, C, D, and F); spores were purified; small molecules were extracted from 10 (A), 6 (B and C), 8 (D), or 5 (E and F) mg (dry weight) of spores; extracts were processed, and NMR spectra were determined as described in Materials and Methods. The peaks due to known compounds (a, arginine; g, glutamate) are labeled, as are peaks due to unknown compounds (the doublet X1 and quadruple peak X2 in spectra A, B, and E and peaks labeled ? in spectra B, C, E, and F). The peaks labeled a? in spectra B, C, and F may be from arginine, but this assignment is not yet definitive. The magnification of spectra B to F has been adjusted slightly to account somewhat for the different amounts of spores extracted (spectra B and C expanded 1.2-fold, spectra D expanded 2-fold, and spectra E and F expanded 1.4-fold.