TABLE 2.
Levels of DPA in DPA-containing spores made with different DPA concentrationsa
| DPA concn (μg/ml) in sporulation medium | % of maximum DPA level (mean ± SD)b in strain:
|
||
|---|---|---|---|
| FB108 (ger3 spoVF) | FB122 (sleB spoVF) | PS4138 (ger3 sleB spoVF) | |
| 3 | 26 ± 15 | 6 ± 11 | 7 ± 8 |
| 6 | 32 ± 15 | 15 ± 20 | 28 ± 25 |
| 10 | 40 ± 20 | 23 ± 16 | 73 ± 33 |
| 25 | 45 ± 20 | 58 ± 22 | 92 ± 23 |
| 50 | 72 ± 25 | ||
| 75 | 95 ± 18 | 91 ± 21 | |
| 125 | 87 ± 24 | ||
| 300 | 100 ± 23 | 100 ± 19 | 100 ± 18 |
a
Spores of various strains sporulated for 1.5 to 3 days with different DPA concentrations in the sporulation medium were purified, and the DPA level in 200 individual spores was determined as described in Materials and Methods.
b
Values are only for those individual spores in populations that actually contained DPA and are expressed as the percentage relative to the DPA level in spores made with 300 μg/ml DPA, with the latter value set at 100%. The absolute values for the DPA levels in the spores of the different strains made with 300 μg/ml DPA were all within 12% of each other and were ∼85% of that in wild-type (PS533) spores.