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. 2008 May 16;190(14):5132–5136. doi: 10.1128/JB.00340-08

TABLE 1.

Kinetic parameters of AcuA^WT and variant AcuA_Bs enzymes

Protein	Peptide substrate				Ac-CoA substrate
Protein	K_m (mM)	V_max (mM min⁻¹ mg⁻¹)	k_cat (s⁻¹)	k_cat/K_m (s⁻¹ M⁻¹)	K_m (mM)	V_max (mM min⁻¹ mg⁻¹)	k_cat (s⁻¹)	k_cat/K_m (s⁻¹ M⁻¹)
AcuA^WT	20 ± 2	0.9 ± 0.02	0.3	1.5 × 10⁴	22 ± 2	0.8 ± 0.03	0.3	1.4 × 10⁴
AcuA^R42H^a	37 ± 5	0.3 ± 0.01	0.1	2.7 × 10³	8 ± 1	0.2 ± 0.01	0.1	1.3 × 10⁴
AcuA^T169A^a	37 ± 5	0.3 ± 0.01	0.1	2.7 × 10³	9 ± 1	0.2 ± 0.01	0.1	1.1 × 10⁴
yGCN5^b	490 ± 80		1.7 ± 0.1	3.5 × l0³	2.5 ± 1.4
P/CAF^c	532 ± 81		2.3 ± 0.07	4.3 × 10³	0.98 ± 0.31

^a

The peptide substrate was not used at saturating concentrations.

^b

Data previously published by others (23).

^c

Data previously published by others (22).