FIG. 6.
Metal ion requirements of WfaP and WfgD. Glucosyltransferase assays were performed as described in Materials and Methods, using 50 mM sucrose as the sonication buffer with 0.25 mM acceptor concentration and without the addition of MnCl2 in the assay. Different metal ions (from 0.1 M stock solutions adjusted to pH 6) were then added to the assays at 5 mM concentrations, as indicated. One hundred percent activity for WfaP corresponded to 1.26 μmol/h/mg and for WfgD to 0.91 μmol/h/mg. Light gray bars, WfaP; black bars, WfgD activity.