FIG. 7.

Dependence of glucosyltransferase activities on buffer pH. Glucosyltransferase assays were performed as described in Materials and Methods, using 50 mM sucrose as the sonication buffer and 0.5 mM acceptor concentration in the assay. Buffers were added at different pH values (MES from pH 5 to 7 and Tris-HCl from pH 7 to 9.5). Activities are indicated as radioactivity of enzyme product per assay. Light gray bars, WfaP; black bars, WfgD activity.