TABLE 1.

Strains and plasmids used in this study

Bacterial strain or plasmid	Description	Source
Strain
G1068	E. coli O56 type strain	—a
G1104	E. coli O152 type strain	—a
G1488	E. coli BL21(DE3) F−ompT hsdSB(rB− mB−) gal dcm(DE3)	Novagen
H1517	E. coli BL21 containing pLW1235	This work
H1514	E. coli BL21 containing pLW1232	This work
H1827	E. coli BL21 containing pLW1448	This work
H1830	E. coli BL21 containing pLW1449	This work
G1370	E. coli DH5α F− φ80lacZ M15 endA recA1 hsdR(rK− mK−)	—b
	supE44 thi-1 gyrA96 relA1 (lacZYA-argF)U169
Plasmid
pGEX-4T-1	GST expression vector, Ampr	Amersham
pET28a+	T7 expression vector, Kanr	Novagen
pLW1232	pGEX-4T-1 containing wfaP at BamHI/EcoRI site	This work
pLW1235	pGEX-4T-1 containing wfgD at BamHI/EcoRI site	This work
pLW1448	pET28a+ containing wfaP at NcoI/EcoRI site	This work
pLW1449	pET28a+ containing wfgD at NcoI/EcoRI site	This work

^aThe Institute of Medical and Veterinary Science, Adelaide, Australia.

^bTianjin Biochip Corporation, Tianjin, China.