TABLE 4.
Acceptor substrate specificities of WfaP and WfgDa
| Compound no. | Concentration (mM) | Acceptor substrate | Activity (%)
|
|
|---|---|---|---|---|
| WfaP | WfgD | |||
| 580 | 0.2 | GlcNAc-PP-PhU | 100 | 100 |
| 604 | 0.2 | GlcNAc-α-PO3-(CH2)11-OPh | 1 | 11 |
| 615 | 0.2 | GlcNAc-α-PO3-PO3-(CH2)6-OPh | 60 | 183 |
| 617 | 0.2 | GlcNAc-α-PO3-PO3-(CH2)16-OPh | 156 | 174 |
| 618 | 0.2 | GlcNAc-α-(CH2)11-OPh | <1 | <1 |
| 619 | 0.2 | GlcNAc-β-(CH2)11-OPh | <1 | <1 |
| 621 | 0.2 | GlcNAc-α-CO-CH2-CO-O-(CH2)11-OPh | <1 | <1 |
| 623 | 0.2 | GlcNAc-α-CO-CH2-PO3-(CH2)11-OPh | <1 | <1 |
| 628 | 0.2 | GlcNAc-α-PO3-PO3-(CH2)9CH3 | 32 | 150 |
| 2 | GlcNAc-β-Bn | <1 | 1 | |
| 612 | 2 | 1-Thio-N-butyrylGlcN-β-(2-naphthyl) | <1 | <1 |
| 580 + 612 | 0.2 + 0.5 | 94 | 96 | |
a
Glucosyltransferase assays were performed as described in Materials and Methods. The compounds indicated were used as acceptor substrates in the standard assays using sonication buffer 1. Ph, phenyl; Bn, benzyl.