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Growth of M. tuberculosis strains in liquid medium. (A and B) The control strain, Glue14 (black squares), and the recombinant strain carrying the mutated glnE allele (pLEDGE3M2) (open diamonds) were grown with 0.1 mM (low) ammonia (A) or 3 mM glutamine (B). (C) The control strain (Glue14 [glnE+int]) (black squares) and the temperature-sensitive strain TS2 (open squares) were grown with 0.1 mM (low) ammonia. Data are the averages and standard deviations from three independent cultures.
