FIG. 5.

Growth of M. tuberculosis strains in the presence or absence of GS inhibition. (A) Wild-type (squares) and Glue21 (ΔglnE) (circles) growth in liquid culture. Strains were grown in 7H9 medium with (open symbols) or without (filled symbols) 200 μM MetSox and 3 mM l-Gln (MSG). Data are the averages and standard deviations from three independent cultures. (B) Long-term survival of wild-type and Glue21 strains. Stationary-phase cultures were kept for 4 months and CFU/ml calculated at each time point. Data are the averages and standard deviations from three independent cultures.