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Active GS in M. tuberculosis strains after removal of MetSox. Strains were grown in 7H9 medium with 200 μM MetSox and 3 mM l-Gln for 7 days, washed, and subcultured in fresh 7H9 medium without supplementation for up to 7 days. Active GS was assayed in cell extracts prepared using the biosynthetic reaction. Data are the averages and standard deviations from three independent cultures, assayed in duplicate (n = 6). GS activity is given in μmol min−1 mg−1.
