Figure 3.
Persistent reduction in PrPSc levels. ScN2a cells were exposed to 1.5 μg/ml: (A) SuperFect, (B) PEI (average-MW ≈60,000), (C) PAMAM, generation 4.0, or (D) no addition. Cells were harvested: lane 1, before addition; lane 2, immediately after 1 wk continuous exposure to test compounds; and lane 3, 3 wk in polyamine-free medium after removal of test compounds. Minus (−) symbol denotes undigested control sample, and plus (+) symbol designates sample subjected to limited proteolysis. Apparent molecular masses based on migration of protein standards are 33.9, 28.8, and 20.5 kDa.