Table 1.
Removal of PrPSc by polymer compounds
| Compound | Molecular weight | Primary NH2 groups | IC50, ng/ml |
|---|---|---|---|
| PAMAM generation 0.0 | 517 | 4 | >10,000 |
| PAMAM generation 1.0 | 1,430 | 8 | >10,000 |
| PAMAM generation 2.0 | 3,256 | 16 | 2,000 |
| PAMAM generation 3.0 | 6,909 | 32 | 400 |
| PAMAM generation 4.0 | 14,215 | 64 | 80 |
| PAMAM-OH generation 4.0 | 14,279 | 0 | >10,000 |
| PPI generation 2.0 | 773 | 8 | 2,000 |
| PPI generation 4.0 | 3,514 | 32 | 80 |
| Low-MW PEI | ∼25,000 | 2,000 | |
| Average-MW PEI | ∼60,000 | 400 | |
| High-MW PEI | ∼800,000 | 80 | |
| Linear PEI | ∼60,000 | 2,000 | |
| poly(l-lysine) | ∼60,000 | >500 | 10,000 |
| SuperFect | 400 |
All compounds were tested at five different concentrations. PrPSc levels were measured by densitometry of Western blot signals.
IC50, approximate concentration of polymer required to reduce PrPSc to 50% of control levels in ScN2a cells after exposure for 16 h.