FIG. 2.

Proteins produced by E. coli strain BLR(DE3) containing a 3.4-kb insert conferring on the bacterium resistance to AC. Protein expression is under control of the T7 promoter. Lanes: (A) uninduced (no isopropyl-β-d-thiogalactopyranoside [IPTG]) cells grown to an absorbance at 600 nm of 0.6; (B) cells induced with IPTG, added initially at an absorbance of 0.6, for 1 hour and reaching an absorbance at 600 nm of 1.3; (C) cells induced with IPTG for 4 h and reaching an absorbance at 600 nm of 2.4. The protein extracts were obtained by boiling whole-cell pellets in SDS and β-mercaptoethanol. The position of the major recombinant protein is shown by the arrow.