FIG. 2.

ygfCBA genes are cotranscribed and expressed mainly in exponential growth under respiration-permissive conditions. Cultures of MG1363 were grown under static, aerobic, or respiration conditions. RNA was prepared from cells harvested at the indicated OD₆₀₀. Northern blotting was performed with a ygfC specific probe. The single major RNA band was estimated at ∼2,300 nucleotides (nt) based on control ladder, as expected for transcription of the three ygf genes as a single operon.