FIG. 1.

Revised annotation of the aar region in A. aceti strain 1023. The ORFs from the original gene assignment (top) were assigned using a kanamycin cassette insertional inactivation at the restriction sites indicated with vertical triangles (16). Insertion points were characterized as resistant to 50 mM acetic acid (open triangles), sensitive (filled triangles), or apparently essential (hatched triangles). The original assigned functions were as follows: aarA, citrate synthase; aarB, unknown; X, unknown; and aarC, possible CoA-transferase or -hydrolase. As of this writing, neither aarB nor X has significant similarity to other inferred protein sequences. The new assigned functions are as follows: orf1, putative metallophosphoesterase; tyrA, chorismate mutase; and sixA, a phosphoprotein phosphatase that affects the ArcA/ArcB two-component regulator. The revised assignments include a single-base insertion in the aarC region that lengthens the ORF slightly. An insertion at the NsiI site, which does not disrupt acetic acid resistance, would disrupt the aarB gene product after Ala19 or would remove or alter seven or fewer C-terminal amino acids in SixA. The scale bar is in units of bp.