FIG. 3.

Kinetic characterization of AarCH6. (A) Acetate saturation data at pH 8.0 (solid circles, LCF assay; open diamonds, VisF assay) and pH 5.0 (solid squares, LCF assay; crosses, VisF assay). The solid lines are fits to each LCF data set, assuming substrate inhibition. The concentration of succinyl-CoA was 0.2 mM. Fitted parameters are given in the text and Table 3. (B) Acetyl-CoA saturation data at pH 8.0 obtained with the LCR assay (solid circles) at 20 mM succinate. The solid line is a fit to the data accounting for competitive inhibition due to CoA (determined in assay mixtures spiked with CoA). The dotted line is a simulation using the fitted parameters at a CoA concentration of 0.