FIG. 2.

Amino acid sequence alignment of TcpH homologues. The TcpH amino acid sequence of pCW3 was aligned with sequences of the equivalent regions from Tn916 (14), pMRS4969, pJIR2774, and pJIR26 (GenBank accession numbers DQ338472, DQ338473, and DQ338471, respectively), encoding CPE toxin production, lincomycin resistance, and tetracycline resistance, respectively (6), and pJGS1495 and pJGS1721, which represent plasmids derived from C. perfringens type C and type D strains encoding β- and ɛ-toxins, respectively (40). Highlighted in the boxed regions are the three conserved sequences: VQQPW, DEREK, and DTK. The symbols below the alignment indicate identical residues (*), strongly similar residues (:), and residues with weak similarities (.). The numbers represent amino acid sequence positions in TcpH from pCW3.