FIG. 5.

Analysis of TcpH interactions by chemical cross-linking and Western blotting. Cell lysates derived from transformants coexpressing His- and GST-tagged TcpH proteins were treated with DTBP (0 or 4 mM, as shown at the top of the gel) and examined by immunoblotting using (A) His-specific antiserum and (B) GST-specific antiserum. R, 150 mM DTT added to produce reducing conditions; NR, nonreducing conditions. The higher-molecular-size protein complex is indicated by the arrow and has a size of >250 kDa.