FIG. 6.

Formation of MBP-TcpH homooligomers. (A) Duplicate samples of purified MBP-TcpH were exposed to increasing concentrations of the chemical cross-linker DTBP. The resultant products then were separated by SDS-5% PAGE gels in the presence and absence of the reducing agent DTT, as shown. The samples then were transferred to nitrocellulose and probed with anti-TcpH antibodies. (B) MBP also was subjected to chemical cross-linking and separated by SDS-12% PAGE, and the products were probed with anti-MBP antibodies. Protein size standards are indicated (in kilodaltons).