FIG. 8.
TcpC interacts with oligomeric MBP-TcpH. Purified MBP (lane 1) and MBP-TcpH (lane 2) were separated on SDS-10% PAGE gels. (A) Coomassie staining of MBP and MBP-TcpH preparations. (B) Western blot of MBP and MBP-TcpH probed with TcpH-specific antibodies. (C) Far-Western blot of membrane preparations containing MBP and MBP-TcpH and then probed with biotin-labeled purified His-TcpCΔ1-98. The protein-protein interaction between His-TcpCΔ1-98 and MBP-TcpH was detected using ExtrAvidin peroxidase (Sigma). Approximate protein sizes are indicated (in kilodaltons). Arrows indicate the MBP-TcpH monomer (M) and oligomer (O).