FIG. 3.

Kinetic behavior of recombinantly produced P. aeruginosa MCCase and GCCase enzymes. (A and B) Carboxylase activities of recombinant LiuB/LiuD (A) and AtuC/AtuF (B) proteins, purified and reconstituted as described in Materials and Methods. (C and D) ATP (C) and bicarbonate (D) concentration dependence of the MCCase and GCCase activities. Data given are the average of three determinations; standard deviations of the given values are shown in panel A, and the averages of two determinations with variations of less than 5% of the given values are shown in panels B, C, and D.