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. 2008 May 16;190(14):4818–4821. doi: 10.1128/JB.00255-08

TABLE 1.

Primers

Name Sequence Restriction site
hmdcln5for GCTGTTGGAATAGACTGCTG
hmdcln5rev GCCCTTATTACTTCTTTTCC
mtdcln5for CGTTTCAGCAGGTTCGAAGG
mtdcln5rev GGGTGTTGCATTAATTGGCG
frcAfor2 GCACCTCTTTAAAAGCTTT
frcArev2 AATGAAACAGCGCCATCTAC
fruAfor CCAGTACTTCAATATCTTTCAC
fruArev TACTTCTTCTGACAACCGAC
hmddel1 AGGCGCGCCACTTTCATATCATACACCTCA AscI
hmddel2 AGGCGCGCCCAATAAAACCTTAAGTATTAC AscI
mtddel1 AGGCGCGCCCATTATATCACCGAAAGATAT AscI
mtddel2 GGGCGCGCCAGAATAAATTTGCATCAAAAT AscI
frcdel1 GGCGCGCCTTACCCATCAGATCACCTATC AscI
frcdel2 GGCGCGCCAATAAATACTGGTGAATCATGC AscI
frudel1 GGCGCGCCACTTTATTCACCTCC AscI
frudel2 GGCGCGCCAATTCTAAATTCCTGAAAAGG AscI
hmddelamp1 ATGGATCCGGCTTGCTGTTGGAATAGAC BamHI
hmddelamp3 TTGGATCCGCCCTTATTACTTCTTTTCC BamHI
mtddelamp1 GAGCTCGGATCCACTAGTAACGGCCGCCAAGTGT BamHI
mtddelamp2 AGAATTGGATCCCGTTTCAGCAGGTTCGAAGGA BamHI
frcdelamp5 ATCTAGAGCACCTTCTTTAAAAGCTTT XbaI
frcdelamp6 CTCTAGAAATGAAACAGCGCCATCTAC XbaI
frudelamp5 TTCTAGACCAGTACTTCAATATCTTTCAC XbaI
frudelamp6 CTCTAGATACTTCTTCTGACAACCGAC XbaI