TABLE 2.
Strains and plasmids
| Name | Feature(s) | Source or reference |
|---|---|---|
| Plasmids | ||
| pCR2.1topo | Ampr Kanr cloning vector | Invitrogen |
| pCRprtneo | hmv-promoter-hpt fusion + Neor cassette in pCR2.1topo | 6 |
| phmdtopo | hmd plus flanking DNA in pCR2.1topo | This study |
| pmtdtopo | mtd plus flanking DNA in pCR2.1topo | This study |
| pfrcAtopo | frcA plus flanking DNA in pCR2.1topo | This study |
| pfruAtopo | fruA plus flanking DNA in pCR2.1topo | This study |
| phmddeltopo | In-frame deletion of hmd in pCR2.1topo | This study |
| pmtddeltopo | In-frame deletion of mtd in pCR2.1topo | This study |
| pfrcAdeltopo | In-frame deletion of frcA in pCR2.1topo | This study |
| pfruAdeltopo | In-frame deletion of fruA in pCR2.1topo | This study |
| pCRprtΔhmdneo | In-frame deletion of hmd in pCRprtneo | This study |
| pCRprtΔmtdneo | In-frame deletion of mtd in pCRprtneo | This study |
| pCRprtΔfrcneo | In-frame deletion of frcA in pCRprtneo | This study |
| pCRprtΔfruneo | In-frame deletion of fruA in pCRprtneo | This study |
| Strains | ||
| Mm900 | M. maripaludis Δhpt | 6 |
| Mm1097 | Mm900 Δhmd | This study |
| Mm1020 | Mm900 Δmtd | This study |
| Mm1183 | Mm900 ΔfrcA | This study |
| Mm1145 | Mm900 ΔfruA | This study |
| Mm1184 | Mm900 ΔfrcA ΔfruA | This study |