FIG. 3.

Pellicle formation. Pellicles were assayed by culturing strains in HMM with Tc in 12-well microtiter dishes for 72 h. A pipette tip was dragged over the air-liquid interface to visualize the pellicle, and Coomassie blue was added to enhance the contrast. (A) Pellicle formation (or lack thereof) by strains carrying the vector, pMAC3, or pCLD29. (B) Pellicle formation by pCLD29-containing syp mutant strains. (C) Pellicle formation by pCLD29-containing vpsR mutant and vpsR syp double mutant strains. Scores were assigned as described in Materials and Methods.