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. 2008 May 9;190(14):4941–4950. doi: 10.1128/JB.00197-08

FIG. 4.

FIG. 4.

Crystal violet staining of glass-attached material following static growth. Glass-attached materials following growth of cells under static conditions were assayed by subculturing into 2 ml HMM with Tc and incubating at room temperature for 48 h and then staining with 1% crystal violet. (A) Crystal violet staining of glass-attached material from cultures of vector-, pMAC3-, and pCLD29-containing cells. (B) Crystal violet staining of glass-attached material from cultures pCLD29-containing syp mutants. (C) Crystal violet staining of glass-attached material from cultures of pCLD29-containing vpsR mutant and vpsR syp double mutant strains. (D) Quantification of the crystal violet-stained material from tubes shown in panels A to C (and additional tubes not shown), solubilized as described in Materials and Methods. Error bars represent standard deviations.