FIG. 5.

Crystal violet staining of glass-attached material following growth with shaking. Glass-attached materials following growth of cells under shaking conditions were assayed by subculturing into 2 ml HMM with Tc and incubating at 28°C for 48 h (A to C) and then staining with 1% crystal violet (D to F). (A and D) Cultures of vector-, pMAC3-, and pCLD29-containing cells. (B and E) Cultures of pCLD29-containing syp mutants, as indicated. (C and F) Cultures of pCLD29-containing vpsR mutant and vpsR syp double mutant strains, as indicated. (G) Quantification of the crystal violet-stained materials from tubes shown in panels D to F (and additional tubes not shown), solubilized as described in Materials and Methods. Error bars represent standard deviations.