FIG. 6.
β-Galactosidase activity. Transcription of the syp locus was monitored using a fusion of the sypA promoter region upstream of a promoterless lacZ gene, inserted in the chromosome at the Tn7 site of wild-type and mutant cells. Cells were grown in HMM with Tc for 24 h. (A) Transcription from PsypA-lacZ in cells carrying the vector control, pMAC3, or pCLD29. (B) Transcription from PsypA-lacZ in pCLD29-containing wild-type (WT), syp, or vpsR mutant cells, as indicated. Error bars represent standard deviations.