FIG. 5.

DAF and α₅β₁ integrin binding properties of DraE and DraD examined by light microscopy of clinical E. coli isolates stained with Giemsa stain. Adherence of E. coli strains to HeLa cells. HeLa cells were incubated with E. coli strains, fixed, stained with Giemsa stain, and subjected to microscopy. ME of E. coli IH11128 (positive control of DraE and DraD adhesion) (A), E. coli DR14 (positive control of DraD adhesion) (B), and E. coli DR14 with a gspD gene knockout (GspD⁻ strain) (negative control for DraE and DraD adhesion) (C). Forty cells associated with bacteria were analyzed by light microscopy. Magnification, ×10,000 (Olympus BX-60 microscope).