. 2008 May 23;190(14):5044–5056. doi: 10.1128/JB.00224-08

TABLE 1.

E. coli strains and plasmids

E. coli strain^a	Plasmid	Mutation	Relevant genotype			Source or reference
E. coli strain^a	Plasmid	Mutation	draC	draD	draE	Source or reference
BL21(λDE3)		ompT lon dcm	−	−	−	20
BL21(λDE3)/gspD		Intron GspD	−	−	−	This work
	pBJN406		+		+	21
	pBJN17	Transposon DraE-TnPhoA	+		−	22
	pBJN16	Transposon DraD-TnPhoA	+	−	+	22
	pBJN417	Transposon DraC-Tn5	−		+	22
	pCC90		+		+	5
	pCC90D54stop	Dr-D54stop	+		−	5
	pCC90DraDmut	ΔdraDstop	+	−	+	35
	pCC90DraCmut	DraC-K15stop	−		+	35
	pInvDsyg-C-His		−		−	35
	pInvDsygstop		−		−	35
DR14		Insertion DraC	−		+	12
DR14/gspD		Insertion DraC, intron GspD	−		+	This work

Symbols separated by the backslash indicate gspD⁺/gspD E. coli strains. All E. coli GspD⁻ mutant strains were complemented in trans with the gspD gene encoded by the pET30-gspD plasmid.