TABLE 2.
Surface expression of a DraD invasin and a DraE adhesin in the tested recombinant E. coli strains with and without the gspD gene knockout
| E. coli straina | Relevant genotype
|
Surface staining of:
|
|||
|---|---|---|---|---|---|
| draC | draD | draE | DraD invasin | DraE adhesin | |
| BL21(λDE3)/gspD | − | − | − | −/− | −/− |
| BL21(λDE3)-pBJN406/gspD | + | + | + | +/− | +/+ |
| BL21(λDE3)-pBJN17/gspD | + | + | − | +/− | −/− |
| BL21(λDE3)-pBJN16/gspD | + | − | + | −/− | +/+ |
| BL21(λDE3)-pBJN417/gspD | − | + | + | +/− | −/− |
| BL21(λDE3)-pCC90/gspD | + | + | + | +/− | +/+ |
| BL21(λDE3)-pCC90D54stop/gspD | + | + | − | +/− | −/− |
| BL21(λDE3)-pCC90DraDmut/gspD | + | − | + | −/− | +/+ |
| BL21(λDE3)-pCC90DraCmut/gspD | − | + | + | +/− | −/− |
| pInvDsyg-C-His | − | + | − | +/− | −/− |
| pInvDsygstop | − | + | − | +/− | −/− |
| DR14/gspD | − | + | + | +/− | −/− |
a
Symbols separated by the backslash indicate gspD+/gspD E. coli strains. All E. coli GspD− mutant strains were complemented in trans with the gspD gene encoded by the pET30-gspD plasmid.