TABLE 3.
Binding phenotype of the analyzed recombinant E. coli strains with and without the gspD gene disruption
| E. coli straina | Relevant genotype
|
Binding phenotype
|
|||
|---|---|---|---|---|---|
| draC | draD | draE | DraD invasinb,c | DraE adhesinc | |
| BL21(λDE3)/gspD | − | − | − | −/− | −/− |
| BL21(λDE3)-pBJN406/gspD | + | + | + | SA+/− | SA+/+SA |
| BL21(λDE3)-pBJN17/gspD | + | + | − | AA+/− | −/− |
| BL21(λDE3)-pBJN16/gspD | + | − | + | −/− | SA+/+SA |
| BL21(λDE3)-pBJN417/gspD | − | + | + | AA+/− | −/− |
| BL21(λDE3)-pCC90/gspD | + | + | + | SA+/− | SA+/+SA |
| BL21(λDE3)-pCC90D54stop/gspD | + | + | − | AA+/− | −/− |
| BL21(λDE3)-pCC90DraDmut/gspD | + | − | + | −/− | SA+/+SA |
| BL21(λDE3)-pCC90DraCmut/gspD | − | + | + | AA+/− | −/− |
| DR14/gspD | − | + | + | AA+/− | −/− |
a
Symbols separated by the backslash indicate gspD+/gspD E. coli strains. All E. coli GspD− mutant strains were complemented in trans with the gspD gene encoded by the pET30-gspD plasmid.
b
AA, aggregative adhesion of DraD.
c
SA, strict adhesion of Dr fimbrial structures (with or without DraD tip subunit) surrounding the HeLa cells.