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Determination of 6-HBs formed by N and C peptides. (A) N-PAGE. (B) Size-exclusion HPLC analysis. (C) Molecular mass of the T21/CP621-652 complex determined by sedimentation equilibrium ultracentrifugation at concentrations of 25 μM in PBS buffer (pH 7.0) at a rotor speed of 33,000 rpm. The observed molecular mass is 24,250 Da (the calculated mass for a trimer is 25,530 Da).
