FIG. 4.

Localization of incoming capsids in the cell. Vero cells were either mock infected or infected with wt HSV-1, ts⁺1249 MR, or ts1249 in the presence of cycloheximide and incubated for 1 (a, c, e, g) or 2 (b, d, f, h) h at 38.5°C. Capsids were detected by indirect immunofluorescence with MAb DM165, specific for VP5, and the secondary IgG antibody FITC-GAM. The nuclei were stained with propidium iodide. The cells were examined by confocal microscopy and digital images taken. Bar, 10 μm.