FIG. 5.

Colocalization of UL25 with VP5 in incoming ts1249 capsids at the NPT. Vero cells were either mock infected or infected with wt HSV-1 or ts1249 in the presence of cycloheximide and incubated for 4 h at 38.5°C. Capsids were detected in the cell by indirect immunofluorescence with MAb DM165, specific for VP5, and the secondary IgG antibody FITC-GAM (a, d, e), and UL25 was identified with rabbit antibody 335 and the secondary IgG antibody Cy5-GAR (b, e, h). Each set of three images shows FITC staining of VP5 (left), Cy5 staining of UL25 (middle), and a merged image of the two for the same field of infected cells (right). The cells were examined by confocal microscopy and digital images taken. Bar, 10 μm.