FIG. 1.
Replication kinetics of HIV-1 NL4-3 and HIV-1 NL4-3Δvif harboring different RT variants in H9 and MT4 cells. Shown are the results of a representative example of three independent experiments. Supernatants from uninfected cells served as negative controls and are indicated by crosses. (A) H9 cells were infected with HIV-1 NL4-3 (black squares), HIV-1 NL4-3M184V (black circles), HIV-1 NL4-3M184I (black diamonds), HIV-1 NL4-3Δvif (open squares), HIV-1 NL4-3ΔvifM184V (open circles), and HIV-1 NL4-3ΔvifM184I (open diamonds). (B) MT4 cells were infected with all six HIV-1 variants listed above, HIV-1 NL4-3K65R+M184V (black triangles), and HIV-1 NL4-3ΔvifK65R+M184V (open triangles). Supernatants were collected daily or every second day and assayed for viral p24 antigen by enzyme-linked immunosorbent assay.