FIG. 8.
Electron microscopic confirmation of virus particle formation and requirement of COOH-terminal NP for incorporation of capsid into virions. (A) Electron microscopic imaging of 293L cells cotransfected with expression vectors encoding Ebola virus NP (upper) or Ebola virus NP 1-450 (lower) with VP40, VP35, and VP24. Arrows indicate the presence of capsid proteins in cross-sections seen with full-length NP but not NP 1-450. (B) Images of 293L cells cotransfected with expression vectors encoding measles virus NP-E (upper) or measles virus NP (lower) with VP40, VP35, and VP24. Arrows indicate the presence of capsid proteins in cross-sections seen with measles virus NP-E chimera but not measles virus NP.